Isolation of Bordetella pertussis from swabs.
نویسندگان
چکیده
Four varieties of swabs held in plastic containers and three swab transport media kits, all obtainable in the United Kingdom, were tested to assess their effectiveness in the isolation of Bordetella pertussis. The highest isolation rates up to 48 hours were obtained with Dacron swabs held in plastic containers. Swab-transport media kits proved unsatisfactory. B pertussis may have a stronger affinity to Dacron than to cotton-wool, and pernasal swabs should be made of Dacron to improve the recovery of B pertussis.
منابع مشابه
Use of supplemented Stainer-Scholte broth for the isolation of Bordetella pertussis from clinical material.
The use of Stainer-Scholte broth supplemented with (2,6-O-dimethyl)beta-cyclodextrin (heptakis) for the isolation of Bordetella pertussis from clinical specimens was evaluated with 3,632 nasal swabs from children and adults with suspected whooping cough or from their family contacts. The liquid enrichment medium was subcultured on charcoal agar with 10% defibrinated horse blood. Charcoal agar a...
متن کاملImpact of nasopharyngeal swab types on detection of Bordetella pertussis by PCR and culture.
A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport syst...
متن کاملVirulence Factors Variation Among Bordetella Pertussis Isolates in Iran
Pertussis is still endemic and the recently resurgence of the disease caused by Bordetella pertussis has been shown in many countries. The polymorphism of the virulence genes of B. pertussis and lack of any information about the allelic variation between the Iranian isolates promotes us to analysis of the genes encoded virulence factors including ptxS1, prn, fim3 and cya to understand the diffe...
متن کاملRegulation of pertussis toxin and lipopolysaccharide levels of Bordetella pertussis 134 in response to modulators
Whooping cough (pertussis) is a highly contagious disease of the human respiratory tract, which is caused by Bordetella pertussis. Reemerge of pertussis in some highly immunized populations and divergency in gene order among several B. pertussis strains promoted this research to study the change of pertussis toxin (PT) and lipopolysacharide levels in response to the different environments. Th...
متن کاملNested duplex PCR to detect Bordetella pertussis and Bordetella parapertussis and its application in diagnosis of pertussis in nonmetropolitan Southeast Queensland, Australia.
A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. No culture-positive-PCR-negative results occurred, giving the method a sensitivity of 100%. For B. pertussis, 58 of 520 patients (11.2%...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- British medical journal
دوره 283 6288 شماره
صفحات -
تاریخ انتشار 1981